FERTILITAS DAN PERSENTASE EMBRIO KERBAU SAMPAI MORULA YANG DIKULTUR DENGAN PENAMBAHAN GLUTATHIONE SECARA IN VITRO
Abstract: The concentration of
high fat content in buffalo embryo culture media is very influential on the
increase in oxidative stress that occurs in conditions of in vitro. The
occurrence of increased oxidative stress in the process of embryo culture in
vitro, would result in lower percentage of embryo culture until the morula
stage (32) cells. The purpose of this study was to determine the percentage of
fertilized oocytes supplemented with glutathione. To know the development of
the embryo until the morula stage (32) cells were cultured in the culture
medium supplemented with glutathione (GSH). To determine the viability of
morula stage embryo or 32 cells. The materials used in this study is ovarian
buffaloes and methods used in this study is an experimental method in the
laboratory. Based on these results it can be concluded that the addition of
glutathione 1.5 mM in media oocyte maturation buffalo in vitro provide a
significant influence on the percentage of maturation ie (P <0:01) or 62.5% and in line
with the high percentage of oocytes matured in treatment increase glutathione
1.5 mM, then the percentage of oocytes were successfully fertilized also higher,
namely 88.98%. Furthermore, the addition of 3 mM glutathione in embryo culture
media in vitro buffalo give a significant influence on the percentage of embryo
development to the morula stage or cell 32, namely (P <0.01) in, or 40.73%.
Keywords: culture, embryo,
buffalo, glutathione, in vitro
Penulis: Harissatria, John
Hendri
Kode Jurnal: jppeternakandd160211