Effectiveness of Brown Algae Extract to Reduce Serum Malondialdehyde and Protein Carbonyl Levels in Streptozotocin-Induced Sprague Dawley Rats
Abstract: Brown algae
(Sargassum duplicatum) contains bioactive compound with natural antioxidant
that may potentially decrease lipid peroxidation and protein oxidation process
in diabetes mellitus. Objective: to prove the effectiveness of brown algae
extract administration (150mg/kgBW; 300mg/kgBW and 450 mg/kGBW respectively) in
reducing serum MDA and PCO levels in streptozotocin-induced sprague dawley rat.
Methods: This experiment study used the post test only control group
design. Twenty eight (28) Sprague dawley rats induced with Streptozotocin (STZ)
40 mg/kgBW (i.p) were divided into four groups (n=7). The first group was
diabetic non treated group (control). The second to fourth groups were the
diabetic rats which given brown algae extract (150mg/kgBW; 300mg/kgBW and 450
mg/kgBW respectively) for 30 days. Serum MDA and PCO levels were examined by
using ELISA method. Data were analyzed using one way ANOVA.
Results: There were no significant differences in serum MDA levels among
groups (p=0,405). However, serum PCO level in group administered with
450mg/kgBW of brown algae extract decreased significantly compared to control
group (p=0.001), group administered with 150mg/kgBW (p=0.001) and 300mg/kgBW
(p=0,037).
Conclusion: Administration of brown algae extract did not decrease serum
MDA levels significantly. Administration of 450mg/kgBW brown algae extract is
effective to decrease serum PCO level significantly.
Keywords: brown algae;
diabetes mellitus; MDA; PCO; Sargassum duplicatum
Author: Lusiana Batubara, Tri
N. Kristina, Banundari Rachmawati
Journal Code: jpkedokterangg160197
