EFFECT OF PURWOCENG EXTRACT ON THE EXPRESSION OF NITRIC OXIDE NEURONAL SYNTHASE IN PENILE TISSUE OF WHITE MALE RATTUS NOVERGICUS
Abstract: To know the effect
of purwoceng extract (Pimpinella alpine) on the expression of neuronal nitric
oxide synthase (nNOS) in the penile tissue of white male rats (Rattus
novergicus) through immunohistochemical examination. Material & Methods:
Animal experiments consisted of 27 male rats, three months of age with a mean
body weight of approximately 200 grams were divided into three groups randomly,
one group consisted of 10 rats. Treatment 1 rats receive Pimpinella alpina
extract with 50 mg/ml, Treatment 2 rats receive Pimpinella alpina extract with
100 mg/ml. In the control group rats receive aquadest as much as 1 ml. Results:
In the control group the average is 75.67, in the treatment group 1 is 113, and
in group 2 is 181.44. The difference in treatment between groups was performed
using one-way ANOVA test. Between control and treatment groups 1 the different
is significant with p value of 0.013 (p<0.05). The mean expression of nNOS
between treatment groups 1 and 2 differ significantly with p=0.00 (p<0.05).
Conclusion: Purwoceng extract (Pimpinella alpine) can increase the expression
of nNOS on NANC nerve fibers (nonadrenergic noncholinergic) in the corpora
cavernosa of the penis that causes smooth muscle relaxation in penile erection.
Keywords: Purwoceng
(Pimpinella alpine); neuronal nitric oxide synthase (nNOS); erection
Author: I Gede Andre Arda
Pratama, H R Danarto
Journal Code: jpkedokterangg180001
