EFFECT OF FLOUR AND PAPAYA LEAF EXTRACT (CaricapapayaL) IN FEED TO AMMONIA CONCENTRATION, VOLATILE FATTY ACIDS AND MICROBIAL PROTEIN SYNTHESIS IN VITRO
Abstract: This research was
conducted to investigate the effect of flour and papaya leaf extract (Carica
papaya L) on a mixed diet of napier grass (Pennisetum purpureum Schaum) and
pollard (Triticumaestivum) to the concentration ammonia, Volatile Fatty Acids
(VFA’s) and microbial protein synthesis invitro. This research method was an
experiment in laboratory with Randomized Block Design (RBD) using 5 treatments
with 3 replications. the treatment were P0 : Control Ration (napier grass +
pollard ratio of 1:1 in the DM), P1 : P0 + 2.0% papaya leaf flour DM, P2 : P0 +
4.0% papaya leaf flour DM, P3 : P0 + extract DM 2.0% papaya leaves, P4 : P0 +
extract DM 4.0 % papaya leaves. The measured variables were concentration of
ammonia incubating rumen fluid 24 hour, concentration of VFA’s and microbial
protein synthesis in the rumen fluid incubation 48 hour. Data were analyzed
using variance (ANOVA), if there any differences in treatment, then continued
with Duncan Multiple Range Test (DMRT). The results of research that provides
highly significant difference (P <0.01) the concentration of ammonia and
microbial protein synthesis, but not significantly (P> 0.05) against the
VFA’s. Conclusion of the research was the additional flour and papaya leaf
extract on blanding of napier grass and pollard can increase the concentration
of ammonia, VFA’s, and microbial protein synthesis. The best treatment is the
addition of papaya leaf flour 4.0 % (P2) because it can increase the
concentration of ammonia and microbial protein synthesis, treatment with the
addition of papaya leaf extract 2.0% (P3) can disposed increase the VFA’s. The
suggestion of this research is that papaya leaf flour 4,0 % (P2) could be added
on the woof because it can improve ammonia concentration and synthesis protein
of microbe, also this is need to do a deep research to know what the effect of
additional of flour and papaya leaf extract on protozoa population based on in
vitro.
Keywords: Papaya leaf,
ammonia, Volatile Fatty Acids, in vitro
Penulis: Pipin Sairullah, Siti
Chuzaemi, Herni Sudarwati
Kode Jurnal: jppeternakandd160151