Cytotoxic activity of simvastatin in T47D breast cancer cell lines and its effect on cyclin D1 expression and apoptosis
Abstract: Statins (HMG-CoA
Inhibitors) is a drug used for decreasing plasma cholesterol levels and used in
therapy to prevent coronary artery disease. Research in animals and
epidemiological studies showed that statin therapy can decrease risk against cancer
associated with cholesterol. Based on that result then research of cytotoxic
activity against simvastatin knowing cultur T47D breast cancer cells and his
influence in decreasing expression of cyclin D1 and induction of apoptosis has
been done.
Research objectives: The aims of this research is to prove activities of
simvastatin against T47D breast cancer cell culture, especially to examine
cytotoxic activity, cyclin D1 expression, and simvastatin effect in apoptotic
induction.
Research method: The type of this research is quasi experiment with using
posttest with non-equivalent control group design. Cytotoxicity test performed
on T47D breast cancer cell cultures using MTT assay to determine IC50 values
after given simvastatine. Expression of cyclin D1 and apoptosis induction test
detected using flow cytometry with antibody monoclonal anti-cyclin D1 and
Annexin V-Pi, then analyzed by FACS-Calibur program.
Results: Simvastatin has cytotoxic effect against T47D breast cancer
cells with IC50 values 25.25 µg/mL. Simvastatin with concentrations of 6.31;
12.62; 25.25 and 50.5 µg/mL was able to decrease the cyclin D1 expression.
Furthermore, simvastatin can induce apoptosis with EC50 values 26.96 µg/mL in
T47D breast cancer cells.
Conclusion: Simvastatin has cytotoxic activity of in T47D breast cancer
cells and decreasing cyclin D1 expression and inducing apoptosis activity in
T47D breast cancer cells.
Keywords: simvastatin,
cytotoxic, cyclin D1, apoptotic, T47D.
Author: Bayu Putra
Journal Code: jpkedokterangg170287
