CHARACTERISTICS OF VIRAL PROTEIN, VP-15, OF WHITESPOT SYNDROME VIRUS ISOLATED FROM INFECTED TIGER SHRIMP Penaeus monodon (Fabricius, 1798)
Abstract: White spot syndrome
virus (WSSV) has caused mass mortality on tiger shrimp (Penaeus monodon)
culture and adversely affects prawn industry worldwide including Indonesia. It
is well known that the protein structure of WSSV plays an important role in the
virus infection and morphogenesis process. A viral protein structure called
VP-15 is located in the nucleocapsid of virion virus. The protein structure
involves in the life cycle of WSSV in host cells. A gene encoding VP-15 could
be involved in constructing the RNA interference (RNAi), so it is needed to
isolate and characterize for RNAi technology purpose. The study was aimed to
isolate and characterize the VP-15 from the infected WSSV tiger shrimp. The
characterization of VP-15 was undertaken through assessment of nucleotide
sequence, amino acid deduction, alignment nucleotide/protein searches using
Genetyx and BLAST program, and dendrogram construction analysis. The results
showed that VP-15 was successfully isolated in form of ORFDNA with a fragment
size of 243 bp. The phylogenetic tree analysis revealed three clusters
corresponding to the time (year) of isolates collection. The VP-15 consisted of
80 amino acids, two start codons (ATG), one stop codon (TAA), and one Kozak
context (AAAATGG). Hydrophilic amino acid was the highest composition (44.2%),
followed by neutral (31.2%) and hydrophobic (24.6%) amino acid groups. The
VP-15 was rich in amino acid of lysine (21.3%), arginine (22.9%) and serine
(24.6%). The successful isolation of VP-15 is a very important step in
providing a basic yet suitable material in constructing the dsRNA vaccine to
control shrimp diseases in aquaculture.
Keywords: Tiger shrimp; WSSV;
VP-15 gene; isolation; characterization
Author: Andi Parenrengi, Alimuddin,
Andi Tenriulo
Jounal Code: jpperikanangg170027