Antibacterial Effect of Pulsatilla chinensis towards Staphylococcus aureus, Shigella dysenteriae, and Salmonella typhi
Abstract: Pulsatilla (P.)
chinensis is a kind of traditional Chinese medicine (TCM) that has
antibacterial effect. It is used to treat diarrhea, dysentery, and other
diseases. The P. chinensis is composed of some potent antibacterial substances
including protoanemonin, saponin, oleanolic acid. The study aimed to determine
the antibacterial effect of P. chinensis towards staphlococcus aureus, Shigella
dysenteriae, and Salmonella typhi.
Methods: This was an experimental descriptive study that was conducted in
2014 using two methods, diffusion and dilution method. In diffusion method, 5
holes were made on the agar that bacteria were growing and different
concentrations of P. chinensis infusion were placed in different hole. The
inhibitory effect was measured by the inhibition zone. In dilution method, 8
test tubes with decreasing concentration of P. chinensis infusion were mixed
with the bacteria suspension and Mueller Hinton Solution. The minimal
inhibitory concentration (MIC) was measured by the last clear test tube. The test
tube with solution that showed absence of bacteria on culture indicated the
minimal bactericidal concentration (MBC).
Results: In diffusion method, P. chinensis infusion showed inhibitory
effect towards S. aureus and bacteriostatic effect towards S. dysenteriae and
S. typhi. In dilution method, there was no antibacterial activity detected.
Conclusions: P. chinensis infusion has inhibitory effect on S. aureus and
bacteriostatic effect on S. dysenteriae and S. typhi.
Keywords: Antibacterial,
infusion, Pulsatilla chinensis, traditional Chinese medicine
Author: Sim Chee Fong, Yanti
Mulyana, Dolvy Girawan
Journal Code: jpkedokterangg160221
