Amino acid diversity on the basis of cytochrome b gene in Kacang and Ettawa Grade goats
Abstract: The objectives of
study were to identify and assess the amino acid diversity of Cytochrome b (Cyt
b) gene, genetic marker and characteristic of specific amino acid in Kacang and
Ettawa Grade goat. Nineteen heads of Kacang goat (KG) and twelve heads of Ettawa
Grade goat (EG) were purposively sampled. The genomic DNA was isolated by
Genomic DNA Mini Kit (Geneaid) and amplified Cyt b using PCR method with
CytbCapF and CytbCapR primers and was sequenced. The results showed that there
were two specific amino acids that distinguish KG and EG goat with C. hircus
and C. aegagrus and four specific amino acids that distinguish KG and EG goat
with C. falconeri, but there were no specific amino acids can be used as a
genetic marker to distinguish between Kacang and EG goat. In conclusion,
specific amino acids in Cyt b gene can be used as a genetic marker among KG and
EG goat with 3 goat others comparator.
Keywords: amino acid;
cytochrome b; genetic marker; local goat
Author: D. A. Lestari, S.
Sutopo, E. Kurnianto
Journal Code: jppeternakangg170036
