Adipose derived stem cell conditioned medium effect on proliferation phase of wound healing in Sprague Dawley rat
Abstract: Disintegration of
skin tissue can lead to disability and death. Recent studies on wound therapy
applied stem cells and adipose derived stem cell conditioned medium (ADSC-CM)
to improve wound healing. However, the role of ADSC-CM in wound healing
mechanism in terms of angiogenesis, quantity of collagen, and epithelialization
is not fully understood. Therefore, this study aimed to analyze the levels of
growth factors (VEGF and EGF) in ADSC-CM and histological features of
angiogenesis, epithelialization, and collagen density after skin incision in
Sprague Dawley rats.
Methods: Thirty rats were injured at the back (full thickness wound) and
treated topically with ADSC-CM, culture medium, basal medium, and without
treatment. Mice were sacrificed on days 3, 7, 14, 21 and 28. After sacrificed,
tissue samples were examined microscopically to assess angiogenesis,
epithelialization, and collagen density. Concentrations of VEGF and EGF in
ADSC-CM were measured by ELISA.
Results: Clinically, wound that was treated with ADSC-CM showed
improvement in wound healing process. ADSC-CM treated wound showed the highest
epithelialization ratio and the fastest wound closure.
Conclusion: There were no statistical significant differences between
groups that were treated with ADSC-CM and not. However, topical ADSC-CM treated
wound revealed a better clinical improvement in epithelialization.
Keywords: ADSC-CM;
angiogenesis; collagen density; epithelialization; wound healing
Author: Twidy Tarcisia, Lia
Damayanti, Radiana D. Antarianto, Yefta Moenadjat, Jeanne A. Pawitan
Journal Code: jpkedokterangg170221
