ADDITION OF ADJUVANTS IN RECOMBINANT SUBUNIT VACCINES FOR THE PREVENTION OF GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV) INFECTION IN HUMPBACK GROUPER, Cromileptes altivelis
Abstract: Infection of grouper
sleepy disease iridovirus (GSDIV) which is a member of Megalocytivirus causes
mass mortalities in marine fish in Indonesia. This study was conducted to know
the effectiveness of recombinant subunit vaccine of GSDIV with an addition of adjuvants
against GSDIV infection. Inactive bacteria Eschericia coli containing
recombinant MCP-GSDIV protein was added with montanide ISA adjuvant at a ratio
of 3:7. The vaccine was administered to humpback grouper, Cromileptes altivelis
by intramuscular and intraperitoneal injection at a dose of 0.1 mL/fish. Test
of the vaccine in humpback grouper was performed in four replicates (four
trials). Results of the vaccination showed that the recombinant protein vaccine
added with the adjuvant increased immunity of humpback grouper, indicated by
higher relative percent survival (RPS= 77.78%) compared to negative control
(PBS) and 50% higher compared to protein control (pET Sumo CAT) at two weeks
post vaccination. The RPS values of the recombinant protein vaccine were still
higher (53.57%-72.73%) than those of the control vaccine and 25%-53.33% of the
protein control in the 4th week post vaccination. GSDIV detection by PCR showed
that MCP-GSDIV-DNA and pET Sumo CAT-DNA were not detected in the vaccinated
fish after one, two, three, and four weeks post vaccination. The fish died in
both of vaccinated and control groups after experimental challenge with GSDIV
were found to be infected with GSDIV. It can be stated that recombinant subunit
vaccine of GSDIV with the addition of montanide ISA adjuvant could be used to
prevent and diminish mortalities of grouper against GSDIV infection.
Keywords: MCP-GSDIV;
Megalocytivirus; sub-unit vaccine; humpback grouper
Author: Ketut Mahardika, Indah
Mastuti, Ahmad Muzaki, Sudewi Sudewi
Journal Code: jpperikanangg160057
