A Comprehensive Study of Chronic Diabetes Complications in Streptozotocin-Induced Diabetic Rat
Abstract: The purpose of this
study was to provide a reference of chronic diabetes complications by
investigating the prolonged hyperglycemia effects on hematological, biochemical
and histopathological changes (liver, kidney, spleen, cardiac muscle, adrenal
gland, and endocrine pancreas) in diabetic rats induced by streptozotocin.
Methods: Ten adult female Sprague-Dawley of uniform age were divided into two
Groups. Group 1 was made diabetic by single intraperitoneal injection of
streptozotocin (60 mg/kg/bw) whereas Group 2 served as control. After six
months, the rats were anaesthetized using pentobarbital. Cardiac puncture was
performed to get 3 ml of the blood sample; following 12 hours of an overnight
fast. Serum chemistry test and complete blood analysis for lipid profile and
blood glucose test; liver and renal functions were performed. Tissue specimens
of liver, kidney, spleen, cardiac muscle, adrenal gland, and endocrine pancreas
were fixed in 10% formal saline and processed for histological study. Results:
There were severe histopathological changes in the affected organs; and the
presence of a significant abnormality of lipid profile, liver, and renal
functions. Conclusions: The presence of histopathological changes with abnormal
biochemical changes is related to the chronic absence of insulin production in
the destroyed β-cells which reflect the diabetic complications in a human
being.
Keywords: biochemical;
histopathological; rats; streptozotocin
Author: Sinan MA Al-Mahmood,
Tariq A Razak, Shahrin TC Abdullah, Nik N Fatnoon NA, Abdul H Mohamed, Imad M
Al-Ani
Journal Code: jpkedokterangg160329
