THE EFFECTS OF CRUDE RECOMBINANT VIRAL PROTEIN VACCINES AGAINST GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV) ON HUMPBACK GROUPER (Cromileptes altivelis)
Abstract: Infection of
Megalocytivirus cause serious mass mortality in marine fish in South East Asian
countries. The aim of this study was to produce recombinant of GSDIV capsid
protein and its protection to humpback grouper Cromileptes altivelis against
grouper sleepy disease iridovirus (GSDIV). A major capsid protein (MCP) was
selected for use as a crude subunit vaccines. This gene target (MCP) was
inserted to the protein expression system vector of pET SUMO and cloned in
cells bacteria Escherichia coli strain BL-21. The MCP was succeded to be
induced using 1 mM of IPTG. Results of protein analysis using MALDI TOF-TOF
indicated that the MCP has measurement of 49.566 kDa with PI index of 6.00, and
contained 453 amino acids. BLAST homology analysis exhibited that the amino
acid sequence of the MCP showed high similarity with MCP of Red Sea Bream
Iridovirus (RSIV). E. coli expressing MCP protein was inactivated using 0.03%
formalin overnight and washed using PBS. The inactivated E. coli as a crude
subunit vaccine was then injected intramuscularly to humpback grouper
juveniles. Subsequently, the juveniles were challenged tested with GSDIV. The
juveniles vaccinated with the MCP recombinant bacteria showed significantly
higher survival rates than control those vaccinated with PBS. Thus, the MCP
fusion protein is considered as a potential vaccine against GSDIV infections in
grouper.
Keywords: humpback grouper;
GSDIV; protein recombinant; vaccine
Author: Ketut Mahardika, Indah
Mastuti
Journal Code: jpperikanangg150060
