THE APPLICATION OF RAPD FINGERPRINTING TO ASSESS INBREEDING LEVELS IN THE CULTURED POPULATIONS OF GIANT FRESHWATER PRAWN, Macrobrachium rosenbergii
Abstract: Inbreeding has been
one of central issues with regard to genetic quality of aquaculture species,
including giant fresh water prawn (GFP). Conventional methods for the
estimation of inbreeding level are available, such as pedigree analyses which
requires a good pedigree record which, unfortunately, is rarely available.
Likewise, microsatellite molecular markers commonly applied to obtain the
coefficient inbreeding estimates are both laborious and expensive. Hence, an
alternative method of inbreeding assessment which is relatively easy but
reliable is in need. This study was aimed to explore the applicability of RAPD
fingerprinting, which is known to be simple and affordable, to estimate
inbreeding level of GFP population. Three GFP populations namely inbred, outbred,
and farm populations with inbreeding level of 25%, 0%, and unknown,
respectively, were genotyped using five polymorphic RAPD primers. The
inbreeding levels mentioned within the first two populations were determined
using pedigree analysis. RAPD banding patterns were then used to calculate band
sharing index (BSI) and inbreeding coefficient (F). Assessment of the
applicability of inbreeding level estimates obtained by RAPD markers was
performed by comparing them to those estimated by pedigree analysis. Results
show that RAPD fingerprinting was capable of delineating populations differing
in their inbreeding coefficients. The pattern resulted from molecular
inbreeding coefficient within the inbred and outbred groups, was congruent with
that shown by pedigree analysis, while the farm population showed closeness to
the inbred group. While the accuracy of the estimate needs to be verified
further, this study suggests that RAPD fingerprinting is applicable to estimate
population inbreeding level, particularly due to its technical simplicity and
cost affordability.
Keywords: inbreeding level;
RAPD fingerprinting; giant freshwater prawn; Macrobrachium rosenbergii
AUTHOR: Imron, Dadan Sunandar,
Rommy Suprapto, Nunuk Listyowati
Journal Code: jpperikanangg100017
