SUSCEPTIBILITY OF DIFFERENT CELLS TO RED SEA BREAM IRIDOVIRUS (RSIV)
Abstract: RSIV is an isolate
virus in the genus Megalocytivirus (family Iridoviridae) that has been reported
to be pathogen in more than 31 marine fish species in East Asia. The aim of
study was to know the susceptibility of several cultured cells to RSIV. RSIV
inoculum was inoculated onto cultured cells and then incubated in 25oC. Routine
observation of cytopatic effect (CPE) was carried out for 7 days and harvested
cells were prepared for virus titration and electron microscopy (EM). The
result showed that RSIV grew and propagated in GF (grunt fin), KF-1 (koi fin)
and BF-2 (barfin flounder) which caused cytophatic effect as cel ls
enlargement. However, RSIV did not propagated on EPC (epithelioma papulosum
cyprini), FHM (feathed minnow) and EK-1 (eel kidney) cells. The virus titer
were 105.3 TCID50/mL in GF cells, 103.8 and 4.3 TCID50/mL in KF-1, 103.6 and
3.8 TCID50/mL in BF-2, and 7 102.1 TCID50/mL in EPC, FHM and EK-1. The EM
observation revealed formation of enlarged cells containing hexagonal virus particles
with 140-160 nm in diameter. These results indicated that GF was cultured cell
to be optimal for replication of isolate RSIV derived from Ise bay, Mie, Japan.
Keywords: RSIV; cells culture;
enlarged cells; virus particles
Author: Ketut Mahardika, Indah
Mastuti
Journal Code: jpperikanangg120030
