SUSCEPTIBILITY OF CYPRINID AND NON-CYPRINID FISH CELL LINES TO KOI HERPESVIRUS (KHV)
Abstract: Koi herpesvirus
(KHV) is an emerging virus that infects koi and common carp (Cyprinus carpio)
with mortality up to 95% within 7 days. The disease is rapidly spreading
worldwide including to Indonesia. However, it has only been documented in koi
and common carp. The aim of this research was to evaluate the susceptibility of
fish cell cultures originated from cyprinid and non-cyprinid fish to KHV. Koi
Fin (KF-1) and Koi Tail (KT-2) cell lines derived from koi carp and SSN-1 cells
originated from fry of striped snakehead were used in this study. The cells
were inoculated with tissue extract of KHV-infected koi carp (experiment 1) and
virus stock of KHV (experiment 2). The cultures were incubated at 22oC and the
onset and type of cytophatic effect (CPE) were observed for 21 days post
inoculation. The results of experiment 1 showed that CPE was observed in KT-2
at day 6 post inoculation. In the experiment 2, however CPE was observed in
KF-1 and KT-2 cells at day 4 post infection. CPE was not observed in SSN-1 of
either experiment 1 or experiment 2. CPE was characterized by extensive
vacuolization of the infected cells. Polymerase chain reaction (PCR) assay of
cell and tissue culture supernatants confirmed that KF-1 and KT-2 showing CPE
were indeed infected with KHV. The results indicated that KF-1 and KT-2 cells
were susceptible and SSN-1 was resistant to KHV. The implication of these
findings was also discussed in the paper.
Keywords: susceptibility; fish
cell culture; KHV; cytophatic effect; PCR
Author: Tuti Sumiati, Agus
Sunarto
Journal Code: jpperikanangg090032