SHORT COMMUNICATION: THE EFFECT OF TANNIN SOLUTION IN PERFORMING SUCCESFULL EGG MICROINJECTION ON AFRICAN CATFISH (Clarias gariepinus)
Abstract: In order to
successfully perform a gene transfer activity using microinjection, an
individual egg is needed. The common standard operating procedure to collect
the individual egg is by applying chemical solution to remove the sticky layer
covering the egg surface. Among several available solutions, tannin is one of
alternative substances that can be used in performing egg microinjection on
African catfish. In this study, the effect of different dosages of tannin
solution to remove the sticky layer of eggs was observed. The study was
conducted at the Laboratory of Fish Breeding and Genetics, Bogor Agricultural
University. Prior to microinjection, the eggs were treated with different
dosages of tannin solution right after fertilization. There were four different
levels of dosage used as the treatments i.e.: 0.3, 0.5, 0.7, and 0.9 ppm. After
the treatments, each individual egg was injected using DNA pmâactin-hrGFP
plasmid with a concentration of 20 μg/mL. The results showed that the dosage of
0.5 ppm of tannin was the most effective treatment compared to the other
dosages. This dosage produced 71.4% hatching rate and 57.1% transgenic embryos
respectively.
Keywords: tannin;
microinjection; gene; transgenic
Author: Muhammad Hunaina
Fariduddin Ath-thar, Komar Sumantadinata, Alimuddin
Journal Code: jpperikanangg090035
