SEQUENCE ANALYSIS OF Megalocytivirus GENOMIC DNA ISOLATED FROM VARIOUS GROUPER AND SEA BASS ON PST1 FRAGMENT AREA
Abstract: Iridovirus have been
detected from mass mortality events on several cultured marine fish species,
namely Cromileptes altivelis (I), Epinephelus fuscoguttatus (II), Plectropomus
leopardus (III), and Lates calcarifer (IV), in Institute for Mariculture
Research and Develepment, Gondol Bali. The aims of this study was to analize
the homology of nucleotide sequences of Megalocytivirus isolated from several
grouper and sea bass. PCR amplification using Pst1 flanking (1-F and 1-R)
region primer set resulted 566-573 base pair. Nucleotide sequence of the
amplicons have 96,2% identity among them. Homology analysis showed that they
were 93%-99% similar to other member of Megalocytivirus. Homology analysis
suggested that isolate were I homolog to III, whereas isolate were II homolog
IV.
Keywords: iridovirus; homology
sequence; Pst1 amplicon
Penulis: Ketut Mahardika,
Indah Mastuti
Kode Jurnal: jpperikanandd160334