Screening of Marine Bacteria Capable of Degrading Various Polyaromatic Hydrocarbons
Abstract: This research aims
to screen and to identify marine bacteria able to degrade Polyaromatic
Hydrocarbons (PAHs). 7 bacterial isolates were screened for the ability to
degrade 6 PAHs (phenanthrene, naphthalene, dibenzothiophene, fluorene,
phenothiazine, and pyrene). This screening step was conducted by sublimation
method, in which the color change or clear zone appearance was used as an
qualitative indicator to show the capability of the selected isolate to degrade
PAHs. Two bacterial isolates, designated as LBF-1-0102 and LBF1-0103, showed
capability in degradating the PAHs tested. In particular, the isolate LBF-1-102
was potential in degrading phenanthrene, naphthalene, dibenzothiphene,
fluorene, phenothiazine, and pyrene.The isolate LBF-1-0103 showed the ability in degrading 2 (two) PAHs,
i.e. dibenzothiophene and fluorene.
Based on partial 16S rDNA analysis, LBF-1-0102 and LBF-1-0103 were identified
as Pseudomonas balerica and Brachybacterium sp., respectively.
Keywords: biodegradation,
marine bacteria, PAH
Author: Elvi Yetti, Ahmad
Thontowi, Yopi Y, Puspita Lisdiyanti
Journal Code: jpperikanangg150032
