Screening of Antitumor Bioactivity of Fungi Associated with Macro Algae and Sponge from Indrayanti Beach, Jogjakarta
Abstract: This research was
aimed to isolate marine derived-fungi which associated with macro algae and
sponge from Indrayanti Beach, Jogjakarta and to screen the antitumor (T47D and
HeLa) bioactivity of the fungi extracts. Three solid media of MEA (malt extract
agar), GPY (glucose peptone yeast) and MFM (minimal fungi medium) were used as
isolation medium. Each of the pure fungi isolates was then cultivated in 100 ml
of liquid medium for 4 weeks at room temperature (27-28°C) in static
conditions. The antitumor activity of the fungi extracts were tested against
breast tumor cells (T47D) and cervical cancer cells (HeLa) using Thiazolyl Blue
Tetrazolium Blue (MTT) assay method. A number of 21 isolates of fungi were
isolated from 4 macro algae and 1 sponge samples. The identification of fungi
isolate was conducted using combination of molecular approach (ITS1-5.8S-ITS4
DNA regions) and macro-micro morphological characteristics. Among those 21
marine fungi species isolated, MFGK-21 extract showed the best anti-servical tumor
(HeLa) with an IC50 value of 240.1 µg/ml and MFGK-27 extract showed the best
anti-breast tumor (T47D) with an IC50 value of 59.6 µg/ml. The MFGK-21 fungi
isolate was identified as Penicillium steckii, while the MFGK-27 fungi isolate
was identified as Aspergillus sydowii.
Keywords: marine
derived-fungi; antitumor; Aspergillus sydowii; Penicillium steckii; Indrayanti
Beach
Author: Nurrahmi Dewi
Fajarningsih, Muhammad Nursid, Ekowati Chasanah
Journal code: jpperikanangg130010
