RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) FINGERPRINTING OF SIX INDONESIAN POPULATIONS OF GIANT FRESHWATER PRAWN, Macrobrachium rosenbergii
Abstract: Indonesia is rich of
giant fresh water prawn (GFP) germ plasms. Best utilization of these resources
for the purpose of either aquaculture development or conservation of genetic
resources requires some information on the structure and levels of their
genetic diversity. This study was aimed to characterize those GFP genetic
resources by applying RAPD genetic markers. Six Indonesian populations of GFP
from Asahan, Barito, Ciasem, Ogan, GImacro and Papua were collected and
analyzed for their genetic variation using five RAPD primers. The results
showed the diversity within the populations, as revealed by the level of
polymorphism, ranged from 29% to 76% while genetic divergence between
populations as shown by genetic distance ranged from 0.04 to 0.50. In terms of
genetic divergence, two genetically distinct groups of GFP, namely the Papua
GFP in one group and the remaining five GFP populations in the other, were
identified. The results also showed the presence of specific population markers
that are useful for genetic identification of GFP populations. Implication of
these finding with regard to breed development is discussed.
Keywords: randomly amplified
polymorphic DNA (RAPD); giant freshwater prawn; molecular markers;
Macrobrachium rosenbergii
Author: Imron, Dadan Sunandar,
Rini Susilowati, Rommy Suprapto, Ikhsan
Journal Code: jpperikanangg090028
