QUANTITATIVE HISTOPATHOLOGICAL ANALYSIS OF ENLARGED CELLS DERIVED FROM HUMPBACK GROUPER, Cromileptes altivelis INFECTED WITH GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV)
Abstract: Pathognomonic sign
of grouper sleepy disease iridovirus (GSDIV) was proposed to be the formation
of enlarged cells and necrotic cells, in which under electron microscope, it is
revealed to be the inclusion body bearing cells (IBCs) and necrotic cells
containing GSDIV viral particles. Spleen and kidney tissues are the major sites
for formation of enlarged cells. This paper described the result of
histopatological analysis of enlarged cells found in the spleen and kidney of
moribund fish after GSDIV challenge. A pathogenicity test was conducted on fish
stocked in two tanks for infected groups and the other two tanks for uninfected
control groups (15 fish per tank). The infected groups were injected
intramuscularly with 0.1 ml of the viral inoculum. The uninfected groups were
injected with the same amount of EMEM-2. The GSDIV-infected humpback grouper
began to die after 6 days post infection and all died after 7 dpi, excluding
one fish which had survived until the end of experimental infection periods
(93% to 100% mortality). All of the diseased fish showed massive formation of
enlarged cells in their spleen, head kidney and trunk kidney. The largest
number of enlarged cells was observed on head kidneys and subsequently followed
by spleens, trunk kidney (2.0-200.3/field of view). This result suggested that
the number of enlarged cells in the affected organs was not the direct factor
that led to the mortality of fish.
Keywords: GSDIV; enlarged
cells; histopathology; quantitative analysis
Author: Indah Mastuti, Yasmina
Nirmala Asih, Ketut Mahardika
Journal Code: jpperikanangg100019
