Purification and Characterization of Transglutaminase from Local Streptomyces Sp. TTA 02 SDS 14
Abstract: Streptomyces sp. TTA
02 SDS 14 is a transglutaminase producing bacteria which previously had
been screened along with more than one
hundred isolates. This research aimed to purify and characterize
transglutaminase from this strain. Transglutaminase was purified from crude
enzyme by ultrafiltration, Q-Sepharose ion exchange chromatography and Sepacryl
S200 size exclusion chromatography sequentially, obtaining yield and
purification fold of 1.36% and 27 folds, respectively. The molecular
weight of the purified transglutaminase was 72 kDa detected by zymogram gel
electrophoresis. The optimum temperature and pH were 50°C and 6. The
transglutaminase was stable at 45°C and could be activated in the presence of 5
mM and 10 mM of Na+, K+, Li+,Ca2+, Mg2+, BPB (4-bromo-phenacyl bromide), and
IAA (iodo acetamide acid), but the activity was inhibited by the presence of Cu+, Zn2+, and PMSF (phenyl
methyl sulfonyl fluoride).
Keywords: Streptomyces sp.,
transglutaminase, purification, characterization, zymogram
Author: Lia Siti Nur'amaliyah,
Dewi Seswita Zilda, Nisa Rachmania Mubarik
Journal Code: jpperikanangg160033