Purification and Characterization of Fucoidan from the Brown Seaweed Sargassum binderi Sonder
Abstract: The brown seaweed
Sargassumsp. is well known as a source of fucoidan. Brown seaweeds found in
Indonesia seas are dominated by Sargassum sp.The objectives of this research
was to purify and characterize fucoidan from S. binderi Sonder. The fresh raw
material was defatted by macerated in methanol:chloroform:water (4:2:1),
filtered, rinsed with acetone and then air-dried in room temperature. The
defatted dried seaweed was extracted with 0.01M HCl pH 4 at room temperature.
Purification has been conducted using anion exchanger. The quality of fucoidan
sample was determined for total sugar, functional group using FTIR, monomer
content, total ash, and total sulfate in the ester form. The result shows that
pure fucoidan contains fucose as the primary sugar component, and other minor
sugars (galactose, glucose, mannose and xylose). Chemical composition of crude
fucoidan consisted of 74.25% fucose; 0.28% uronate acid; 10.29% sulfate and
5.5% protein. Purification using DEAE Sephadex A-25 gave 4 fraction pools
yielding total sugar (%) of F1 (17.59); F2 (18.92); F3 (13.72); F4 (49.76),
respectively. The components that build the fucoidan of S. binderi Sonder were
estimated derived to be from fucoidan oligomers including (1,4)-L-FucS-Gal and
D-(1,4)-Gal-GalS.
Keywords: Fucoidan, Sargassum binderi Sonder, brown seaweed,
purification
Author: ellya sinurat,
Rosmawaty Peranginangin, Endang Saepudin
Journal Code: jpperikanangg150038
