POTENCY AND EFFICACY TEST OF A VACCINE IN ADDITION WITH ADJUVANT AGAINST KOI HERPESVIRUS IN KOI (Cyprinus carpio)
Abstract: Koi Herpesvirus
(KHV) is a malignant virus infecting the goldfish and koi in all stadia and
cause mortality up to 95%. The purpose of this study was to determine the
potency and efficacy of inactivated-vaccine in addition with adjuvant against
KHV in koi fish. The viral propagation was done using a KF-1 cell line in 25
cm3 flask. The cultured virus was harvested on 12 days post inoculation, and
then the harvested virus was inactivated with 0.1% formalin as
inactivated-vaccine. Three hundred of test fish (10.38 ± 1.25 g) maintained in
126 L of plastic containers with aeration, and fed with pellets twice a day.
After 14 days of adaptation, the fish were divided into five treatments (A=
vaccine; B= vaccine + Complete Freund’s Adjuvant; C= vaccine + Incomplete
Freund’s Adjuvant; K+= positive control, and K-= negative control) and each
treatment has four replicates. Vaccine was given by injecting intramuscularly
of 0.1 mL per fish. All fish were challenged by injecting intramuscularly of
0.1 mL of KHV virus with concentration of 104.58 TCID50/mL after 21 days post
vaccination. The results showed that the B treatment had higher (P<0.05)
values of hematocrit level, lysozyme activity, and titer of antibody compared
with positive control. In addition, the survival of fish in B treatment also
had the highest percentages and significantly different compared to other
treatments (P<0.05). The conclusion of this research was the application of
inactivated KHV vaccine in 0.1% formalin with the addition of Complete Freund’s
Adjuvant through the injection dose 0.1 mL fish-1 in 104.58 TCID50/mL capable
to enhance the immune responses and raised the optimal protection of KHV
antibody in koi fish.
Keywords: inactivated
vaccines; adjuvants; immune response; Cyprinus carpio; KHV
Author: Lili Sholichah, Munti
Yuhana, Angela Mariana Lusiastuti, Tri Heru Prihadi
Journal Code: jpperikanangg160052
