MOLECULAR IDENTIFICATION OF SPONGES OBTAINED FROM SERIBU ISLANDS NATIONAL PARK AND THEIR ASSOCIATED BACTERIA
Abstract: Sponges are simple
multicellular animals that produced many pharmaceutical secondary metabolites.
Some sponge-associated bacteria are proven to produce the same metabolites as
their host, giving an opportunity to mass produce the potential metabolites. The
aim of this research was to analyze the diversity of sponge-associated bacteria
and to identify the host sponge. Samples
were collected from Seribu Islands National Park. Partial identification of
sponges were conducted by molecular technique with the mitochondrial cytochrome
oxidase subunit 1 (CO1) as the target area. The diversity of sponge-associated
bacteria was determined by Terminal Restriction Fragment Length Polymorphism
(T-RFLP) method. Result showed that sponges PS-17-12 has similarity with Petrosia
sp., while PS-26-12 and PS-38-12 has similarity with Xestospongia muta. From
the 3 sponge samples, 85 species of bacteria was obtained which can be
classified into 9 phylums and 1 uncultured bacteria/environment sample. Some of the sponge-associated bacteria identified
were known as a potential producer of metabolites with antibiotic activity.
Keywords: sponge, bacterial
diversity, T-RFLP
Author: Gintung Patantis,
Gemilang Rahmadara, Dewi Elfidasari, Ekowati Chasanah
Journal Code: jpperikanangg130016
