MARINE SPONGE Aaptos suberitoides, IT’S POTENTIAL SOURCE OF NATURAL ANTIBACTERIAL FOR CONTROLLING Vibrio harveyi ON TIGER SHRIMP (Penaeus monodon) CULTURE
Abstract: The study aims to
isolate and identify the natural antibacterial compounds potential from Aaptos
suberitoides for Vibrio harveyi control on tiger shrimp (Penaeus monodon)
culture. The agar diffusion method using paper discs was used to determine the
antibacterial activity of extracts (diethyl ether (DEE), butanol (BUE) and
aqueous (HOE) and compounds successfully isolated against Vibrio harveyi.
Findings showed that the antibacterial activity was concentrated in BUE with
the inhibition zone of 17.2±0.1 mm. Meanwhile, two other extracts (DEE and HOE)
did not exhibit any antibacterial activity against V. harveyi. From the active
BUE, it was successfully isolated two compounds giving a strong anti-vibrio
activity with the inhibition zone of 22±0.1 mm. The IR, 1H, 13C, COSY, HMQC,
HMBC, and MS spectrum analysis indicated that both active compounds identified
as aaptamine (1) and 9-demethyloxyaaptamine (2). The study suggested that
marine sponge A. suberitoides may have potential compounds source for
controlling of V. harveyi on tiger shrimp culture.
Keywords: Aaptos suberitoides;
aaptamine; 9-demethylaaptamine; Vibrio harveyi; Penaeus monodon
Author: Rosmiati, Andi
Parenrengi, Emma Suryati
Journal Code: jpperikanangg150040
