IDENTIFIKASI BAKTERI PATOGEN PADA IKAN NILA (OREOCHROMIS NILOTICUS) DI LOKASI BUDIDAYA IKAN AIR TAWAR KABUPATEN KEPULAUAN SANGIHE
ABSTRACT: This research aimed
to identify pathogenic bacteria that infected nile tilapia (Oreochromis
niloticus) cultivated at five Districts in Tahuna Regency including Tamako,
Kecamatan, Tabukan Tengah, Tabukan Utara,
Manganitu and Tahuna Districts, and to analyze water quality
(temperature and pH). The research
was conducted from July to August
2017. Isolation and identification of
bacteria was performed at the Test
Laboratory, Station of Fish Guarantee, Quality Control and Fisheries Product
Safety Grade II, Tahuna. Target organ examined was kidney. The bacteria was grown on TSA and then
isolated. The bacteria was identify by gram stain, motility, aerobic, catalase,
oxidase, O/F, glucose and Rimler shots for Aeromonas hydrophila. The bacteria
was then identified using Manual for the Identification of Medical Bacteria. From the 30 samples identified, it was found
seven pathogenic bacteria infected fish namely A. hydrophila observed at 11
fish (36.6%), Corynebacterium sp. observed at 6 individuals or 20 %,
Enterobacteria sp. obtained at 5 fish or 16,6 %, Listeria sp. at 2 fish or 6,6 %, Pseudomonas sp. at 1 fish
or 3,3 %, Plesiomonas sp. at 1 fish or 3,3 % and Kurtiha sp. at 1 fish. Water quality parameters at all five
districts ranged between 25 and 28° C while pH ranging from 6- 7 and suitable based on the PP N9. 42
Tahun 2001.
Keywords: pathogenic bacteria, aquaculture, Oreochromis
niloticus
Penulis: Usy N. Manurung,
Darna Susantie
Kode Jurnal: jpperikanandd170414
