IDENTIFICATION AND CULTIVATION OF MFW 23-08 ISOLATED FROM MARINE SPONGES FOR BIOACTIVE COMPOUND PRODUCTION
Abstract: Production of marine
bioactive compound for commercial usage has been hampered due tothe problem of
raw material supply. To overcome this, marine microbes especially those
associatedwith the bioactive-compound producer biota, has been explored as
bioactive sources, with severaladvantages such as shorter production time,
cheaper production cost and avoiding over exploitationof marine biota sources.
Previous research showed that fungi MFW 23-08 was one of the potentialisolates
from Wakatobi sponges which produced bioactive compounds that was active
againstbreast cancer cell line and as antioxidant. This study was intended to
identify MFW 23-08 andoptimize the production of its bioactive compound through
optimization of MFW 23-08 culture.Culture optimization was conducted using 3
liquid media, i.e. malt extract broth (MEB), glucosepeptone yeast (GPY), and
minimal fungal media (MFM), and cultivation periods, i.e. 2, 4, 6, 8, and10 wk.
Results revealed that MFW 23-08 crude extract of 2 wk-MFM cultivation, at the concentrationof
30 μg/ml, was able to retard 87% breast cancer (T47D) cell growth. While at
concentration of100 μg/ml, the 6 wk. MEB cultivated extract was able to hamper
free radicals (56%). However, thecrude extract from MFM media cultivation, in
the concentration of 50 and 100 μg/ml was not able toinhibit Escherichia coli
and Staphylococcus aureus growth. Based on molecular identificationusing
ITS1-ITS4 primers, MFW 23-08 isolate was 99% similar to Penicillium citrinum, P.griseofolvum and
Penicillium sp.
Keywords: bioactive, fungi,
sponge, MFW 23-08, breast cancer cell
Author: Ekowati Chasanah, Asri
Pratitis, Wibowo Mangunwardoyo
Journal Code: jpperikanangg120018
