GENETIC VARIABILITY AND POPULATION STRUCTURE OF GROUPER (Epinephelus suillus) FROM MAKASSAR STRAIT AND BONE BAY, SOUTH SULAWESI, INDONESIA
Abstract: Random Amplified
Polymorphic DNA (RAPD) was employed to determine the genetic variability and
population structure of grouper (Epinephelus suillus) from Makassar Strait and
Bone Bay, South Sulawesi, Indonesia. Genomic DNA was isolated from preserved
muscle tissue using Phenol-Chloroform technique. Among 24-screened arbitrary
primers, ten primers (OPA-02, OPA-06, OPA-08, OPA-10, OPA-15, OPA-16, OPA-17,
OPA-18, OPA-19 and CA-05) were selected to generate RAPD fingerprinting of
grouper populations. The ten primers generated a total of 212 fragments (loci)
and 120 polymorphic fragments in their size ranging from 250 to 2,500bp. The
high polymorphism (60%) was obtained from Makassar population followed by Bone
(59%) and Pare-Pare populations (50%). Similarity index of individuals was
0.86±0.07 for Pare-Pare, 0.80±0.11 for Makassar and 0.82±0.07 for Bone
population. Fifteen fragments from ten primes were identified as
species-specific markers of E. suillus. The UPGMA cluster analysis showed that
the dendrogram seemed to be clustered according to their geographical location,
where Pare-Pare population was genetically closer to Makassar population
(D=0.20) than to Bone population (D=0.24).
Keywords: genetic variability;
population structure; RAPD finger printing; grouper
Author: Andi Parenrengi, Andi
Tenriulo
Journal Code: jpperikanangg080009
