GENETIC, COLORATION, AND GROWTH PERFORMANCE OF TWO DIFFERENT VARIETIES OF Kappaphycus alvarezii
Abstract: Two different colors
(green and brown) of Kappaphycus alvarezii have been farmed in Indonesian
waters for many years. This study aimed at comparing two ‘varieties’, i.e.
green and brown, both genetically and morphologically. Samples for DNA analysis
were collected from a farmer in Pinrang Regency, South Sulawesi. Five universal
primers i.e. Ca-01, Ca-02, P-40, P-50, and DALRP were selected to obtain DNA
genetic markers in differentiating the green and brown varieties. To compare
coloration patterns during cultivation and the growth performance of both
varieties, a field experiment was performed in a seaweed farming area in
Pinrang Regency, during dry season of August-September 2004. The result of
genetic assessment showed that the five selected primers revealed different
RAPD banding pattern for both varieties. P-50 and DALRP primers demonstrated
the greatest amplification in differentiating RAPD fragment between green and
brown varieties. Fragment 900 bp and 1.300 bp were consistently generated in
the green variety but were not amplified in the brown variety. The result of
the field study confirmed that the coloration pattern of green and brown
varieties was fixed; no interchange in color occurred during one crop
cultivation.
Keywords: genetic; morphology;
Kappaphycus alvarezii
Author: Sulaeman, Andi
Parenrengi, Emma Suryati, Rosmiati
Journal Code: jpperikanangg070008