FLUCTUATION OF POST-PRANDIAL PLASMA MINERAL LEVEL OF JUVENILE JAPANESE FLOUNDER, Paralichthys olivaceus FED DIETARY PHOSPHORUS AND PHYTASE SUPPLEMENTATION
Abstract: In order to
investigate the phytic acid degradation in the gut of post juvenile Japanese
flounder, indirect method was carried out by measuring the pre-prandial and
postprandial plasma mineral and alkaline phosphatase (ALP) level as well as
liver phosphorus content. The experiment was designed into a Randomized Block
in which experiment units were grouped according to sampling days at 10, 20 and
30 days of feeding time. Experimental diets contained three levels of dietary
inorganic phosphorus at 0.0; 0.25 and 0.5% combined with two levels of dietary
phytase at 0 and 2,000 FTU/kg diet. Juvenile Japanese flounder (IBW = 36.2 g)
were randomly distributed into 6 tanks of a 200 L capacity with density of 15
fish/tank. Blood sampling was carried out at 0 hour (before feeding or
pre-prandial) and at 1, 3, 6 and 12 hour post feeding (post-prandial) time in
three sampling days, respectively. Plasma was measured for mineral and ALP
levels, while liver was analyzed for P content. The observation showed that
fish fed without both dietary IP and phytase supplements had the lowest
postprandial plasma IP, Mg and ALP levels during 12-h postprandial period.
Plasma IP level at 6-h post-feeding in groups fed dietary 0.25 and 0.5% IP were
significant higher when diet supplemented with phytase than those without
phytase supplement. Peak level of plasma IP in fish fed 0.25% IP was similar to
fish fed 0.5% with the presence of dietary phytase. At 1 and 3-h post-feeding,
plasma Ca level increased in all groups, but significant difference was only
observed between group fed diet without both dietary IP and phytase and other
groups. Similar to plasma IP level, peak of plasma Mg and ALP concentration
occurred in fish fed 0.25% IP together with phytase, and did not significantly
differ from fish fed with 0.5% IP even when phytase was included in diet.
Keywords: phytase; phosphorus;
mineral; alkaline phosphatase; Japanese flounder
Author: Asda Laining, Rachmansyah,
Lideman, Shunsuke Koshio
Journal Code: pperikanangg100022