EVALUATION OF GENETIC VARIABILITY OF FRESHWATER PRAWN COLLECTED FROM MAKASSAR-SULAWESI, PANGKALANBUNKALIMANTAN, JAMBI-SUMATRA, SUKABUMI-JAVA, AND GIMacro USING mtDNA CO-I MARKERS
Abstract: The objective of
this research is to evaluate the genetic variability of freshwater prawn,
Macrobrachium rosenbergii. The genetic variability of freshwater prawn
collected from Makassar-Sulawesi, Pangkalanbun-Kalimantan, Jambi-Sumatra,
Sukabumi-Java, and GIMacro strain was examined using polymorphism of the
mitochondria DNA (mtDNA) markers. Twelve composite haplotypes were detected
following digestion of CO1 sequences with four endonucleases: Hae III, Rsa I,
Mbo I, and Taq I. The average haplotype diversity was 0.217. Significant
genetic difference was observed among freshwater prawn populations, especially
among Makassar-Sulawesi population and others. Makassar-Sulawesi strain has
future prospect for genetic resources in breeding program.
Keywords: genetic variability;
freshwater prawn; mtDNA CO-I
Author: Estu Nugroho, Mulyasari,
Anang Hari Kristanto, Fauzan Ali, Gunawan Gunawan
Journal Code: jpperikanangg080003
