EFFICACY OF GP-11 KHV DNA VACCINE IN Cyprinus carpio haematopterus
Abstract: Koi herpesvirus (KHV)
is one of the major pathogen for koi and common carp which cause high mortality
and economic losses for the farmer. The purpose of this study was to determine
the efficacy of glycoprotein-11 (GP-11) KHV DNA vaccine and compared to GP-25
KHV DNA vaccine. The vaccine in the form of naked DNA plasmidwas delivered by
intramuscularly injection to the 3-month-old koi. The fish were divided into
six groups, i.e. unvaccinated group (negative control C- and positive control C+), and vaccinated group
(2.5 μg/100 μl of GP-11 (group 1), 7.5 μg/100 μl of GP-11 (group 2), 12.5
μg/100 μl of GP-11 (group 3), and 12.5 μg/100 μl of GP-25 (group 4)). At day 42
post vaccination, all fish of each groups were challenged by injecting KHV
titre 10-3 FID50. Number of dead fish was counted everyday after the challenge
until 30 days. The results showed that vaccinated fish were had survival rate
of 83.33-93.33% (group 2, 3 and 4). It’s show that GP-11 KHV DNA vaccine has
high efficacy. As conclusion, GP-11 DNA vaccine could be an alternative DNA
vaccine for preventing KHV infection.
Keywords: Cyprinus carpio koi;
DNA vaccine; injection; koi herpesvirusa
Author: Sekar Ayu Chairunnisa,
Sri Nuryati, Alimuddin, Sri Murtini, Ayi Santika, Dwi Hany Yanti
Jounal Code: jpperikanangg160041
