DNA BARCODING OF FRESHWATER FISH SPECIES OF MANNA RIVER (BENGKULU) AND SEMANKA RIVER (LAMPUNG)
Abstract: The identification
of fish species is challenging. DNA barcoding provides new perspective in
ecology and systematics of fishes. In this study, we assess intraspecific and
interspecific genetic divergence among several freshwater fish species from
Manna River of Bengkulu Province and Semanka River of Lampung Province, using
data from mitochondrial cytochrome c oxidase subunit I (COI) gene. The results
show that a total of six freshwater fish species identified in this study
exhibited high levels of interspecific variation based on 51 analyzed
sequences. Almost all species corresponded to a single species, cohesive array
of barcode sequences distinct from other species. Based on Kimura Two
Parameters (K2P) within species, mean pairwise sequence distances comparisons
range from 0.3 to 6.3%, while interspecific comparisons were much higher range
between 1.7 to 29.4%, except species Tor macrolepis and Tor putitora showed
high levels of genetic similarities and no reciprocal monophyly. Using this
method will obviously allow the identification eggs, larvae, fillets and fins
of freshwater fish.
Keywords: Molecular; fish;
COI; Manna; Semanka
Author: Arif Wibowo, Achmad
Farajalah, Husnah
Journal Code: jpperikanangg130033
