CYTOTOXIC SATURATED FATTY ACIDS FROM THE INDONESIAN SEA CUCUMBER Holothuria sp.
Abstract: Sea cucumbers are
one of Indonesia’s marine organism with the potential to be developed as
possible herbal medicines. In a preliminary study of cytotoxic activity of
ethanol extracts of 14Indonesian sea cucumber species, the most active extract
came from the Holothuria sp. Thecurrent research aimed to identify the major
cytotoxic component in the sea cucumber Holothuriasp., that yielded the most
active extract. The samples were collected from South Lampung beach.Isolation
of the cytotoxic component was done employing liquid flash and preparative
reversedphase (C 18) chromatography. Cytotoxic ity evalu atio n of fractio ns
collected durin g thechromatography was conducted using the MCF-7 tumor cell
line. Elucidation of the structure ofmost active isolate was done by NMR
(Nuclear Magnetic Resonance) spectroscopy and GC-FID(Gas-Chromatography-Flame
Ionisation Detector) analysis. The results of these analyses showedthe most
active compound to be stearic acid; IC 50towards MCF-7 cells 10.32 ppm.
Keywords: Holothuria sp.,
cytotoxicity, MCF-7, stearic acid
Author: Hedi Indra Januar,
Muhammad Nursid, Ekowati Chasanah
Journal Code: jpperikanangg140020
