BACTERIAL DIVERSITY OF THE DEEP SEA OF SANGIHE TALAUD, SULAWESI
Abstract: Deep sea is an
extreme environment characterized by cold temperature, high pressure,
lackof light and nutrients.
Microorganisms live in these habitat are unique microorganisms andknown to have
tremendous source of potential agents for biotechnology processes. Indonesia
asan archipelagic country has a vast deep ocean. This study aims to see the
diversity of bacteria inSangihe Talaud Deep Sea, Sulawesi. Analysis of
bacterial diversity was carried out by culturedand uncultured method. Terminal
Restriction Fragment Length Polymorphism (T-RFLP) techniquewas used for
uncultured analysis of the microorganisms biodiversity, while cultured one
wasdone by plating the samples of water onto Zobell media. The results showed
that, there were 21isolates obtained by cultured method. The identification
which based on 16S rDNA by PCR methodshowed the genus of Pseudomonas,
Pseudoalteromonas, Alteromonas, Vibrio, Shewanella andUncultured bacterium were
identified. However, 14 classes of bacteria were obtained by usingTRFLP method
i.e Acetobacteraceae class, Actinobacteria, α-proteobacteria, -proteobacteria, δ-proteobacteria, γ-proteobacteria, Bacili,
Bacteroidetes, Chlorobi, Chroococcales, Clostridia,Erysipelotrichi,
Synergistia, and Zetaproteobacteria. here were also unclassified bacteria anduncultured
bacterium found in the samples.
Keywords: deep sea, diversity,
bacteria, PCR, T-RFLP, Sangihe Talaud
Author: Gintung Patantis,
Ekowati Chasanah, Dewi Seswita Zilda, Ikhsan B. Waluyo
Journal Code: jpperikanangg120015
