VARIASI GENETIK GEN MYOSTATIN EKSON 3 PADA SEMBILAN BANGSA KAMBING LOKAL DI INDONESIA
ABSTRACT: Myostatin gene plays
a role in helping to control the growth and development of muscle tissue.
Identification of genetic diversity in nine local goat breeds in Indonesia has
done. The aim of this study was to obtain information myostatin gene diversity
of exon 3 in local goats in Indonesia. The total of 10 samples was selected
from 80 samples of goat's blood collected comprising each sample of the
population of goats breeds, i.e., Samosir, Muara, Kacang, Costa, Peranakan
Etawah, Boerawa, Gembrong, Boer, and Boerka goats. The gene diversity and
nucleotide base changes were identified by using polymerase chain reaction (PCR)
and sequencing techniques. The analysis showed that there is eight variant
identified in appropriate with those found in sequencing results. Deletion
variations were found in Costa and Samosir goats in T552- and G560-.
Substitution variations were found in Gembrong (A7C & A11T), Peranakan
Etawah (T10A & A11T), Burawa (T10A & A11T), Muara (A11T), Samosir
(A11T), and Boerka (A182T, T437A, T439A, & A445G). Variations on the
chromatogram peak overlapping contained in the base position to 13. Analysis of
variance showed that there was a special mutation in exon 3 that affects the
amino acid tyrosine into lysine. Variants were found in nine goat breeds
associated with phylogenetic and genetic distance of goats, Boerka goat has the
highest level of genetic variation, it indicated that Boerka goat was
crossbreed
Penulis: Rissa Herawati
Ginting, Achmad Farajallah, Dyah Perwitasari Farajallah, Aron Batubara
Kode Jurnal: jppertaniandd170503
