UJI AKTIVITAS ENZIM SELULOLITIK DARI BEKICOT (Achatina fulica) PADA BEBERAPA SUBSTRAT LIMBAH PERTANIAN
Abstract: The purpose of this
study to determine interaction of some agricultural waste substrate with
incubation time using crude extract of cellulolytic enzyme extracted from snail
(Achatina fulica). This study used a randomized block design (RAK) in factorial
pattern with two factors. The first factor was the time (W) incubation with four
levels. The second factor was the type of substrate, namely agricultural wastes
(L) which comprises four types. From both these factors retrieved 16 treatment
and each treatment was repeated three times, so the retrieved data 48 units of
the experiment. Data were analyzed statistically using ANOVA (Analysis of
Variance). If F counted was greater or equal than to F table then the analysis
continued by Duncan's New Multiple Range Test (DNMRT) at 5% level. The results
of the protein crude extract of cellulolytic enzyme 1.712 mg/ml enzyme. The
results of the highest glucose levels on a banana peel waste substrate
interaction with the incubation time 180 minutes is 457.00 mg/100 ml. The
highest enzyme activity result in the interaction of banana peels waste
substrate with incubation time 180 minutes is 0.45 U/ml.
Keywords: Cellulolytic enzyme
crude extract of snail (Achatina fulica), agricultural waste substrate, and
incubation time
Penulis: Muhammad Akbar
Hasibuan, Fajar Restuhadi, Evy Rossi
Kode Jurnal: jppertaniandd170300
