PRODUKSI IGY SPESIFIK STAPHYLOCOCCUS AUREUS DARI ISOLAT ASAL KASUS STAPHYLOCOCCOSIS PADA KELINCI
ABSTRACT: This experiment aims
to produce immunoglobulin Y (Ig-Y) Staphylococcus aureus isolates origin
staphylococcosis case in rabbits. Vaccination is done several times using
Staphylococcus aureus active in 109 cfu/ml. Injecting the chicken is done four
weeks in a row, the first week is done by intravenous injection of S. aureus
antigens, the second week of the injection sub cutan S. aureus emulsified with
Freund's complete adjuvant, followed by the third and fourth week of the
injection of S. aureus antigen emulsified with incomplete Freund's adjuvant.
Egg samples were taken after four weeks of the last injection for
identification, purification, and determination of specific IgY against S.
aureus in egg yolk. IgY specificity qualitatively tests performed by the AGP
test (To Gel Precipitation). IgY extraction is done using PEG - Ammonium
sulfate, purified IgY concentration calculated by the method of Bradford. The
results showed that IgY began to be detected in egg yolk at week six after
immunization, with the average levels of IgY is 1.7 mg/ml.
Penulis: Triwardhani
Cahyaningsih, Fachriyan Hasmi Pasaribu, Agustin Indrawati
Kode Jurnal: jppertaniandd170470
