PENGARUH METODE STERILISASI DAN KONSENTRASI FILTRAT BAKTERI ENDOFIT TANAMAN TEBU UNTUK MENGENDALIKAN PENYAKIT BLENDOK (Xanthomonas albilineans (Ashby) Savulescu 1947)
ABSTRACT: Leaf scald
(Xanthomonas albilineans) is an important pathogen of sugar cane in Indonesia
and cause significant losses at several main sugarcane plantation. Some control
measures, e.i. bactericides, smeared lysol 20% or alcohol 70% on cutting knife
seedling for disinfectant, and hot water treatment at 50 minutes on 52,5ᵒC,
have not given satisfactory result in managing this leaf scald in sugarcane on
the field. Biological control with filtrate of endophyts bacterial were
alternative to biocontrol agents Xanthomonas albilineans. The objectives of
this research were to determine: influence of sterilization methods and endophytic bacterial filtrate
dosages of sugarcane to control leaf scald (Xanthomonas albilineans). This
research was conducted in Pyhtopathology Laboratory at Faculty of Agriculture
University of North Sumatra from April until December 2014. This research had
done with Completely Randomized Design (CRD) with three factor: 1. type of
endophytic bacterial filtrates: filtrate of B2, B7 and B9 Sterilization methods of endophytic bacterial filtrate:
control (no sterilization), Sterilization by autoclave and Sterilization by
filter membrane, 3. Dosage of endophytic filtrate with four level: 0,1%, 0,01%,
0,001% and 0,0001%. The result showed: 10 isolate endophytic bacteria had
exploration were: B1, B2, B3, B4, B5, B6, B7, B8, B9, and B10. Three isolate
had clear zone on petridish ≥ 20 mm in
vitro testing is B9, B7, and B2. The results showed that the membrane filter
sterilization method was better when compared to autoclave sterilization,
namely: the highest inhibitory zone diameter of endophytic bacteria in the
treatment of B9 filtrate with a membrane filter sterilization method 0,1% at 3
day after inoculation that was equal to 23.00 mm.
Penulis: Astri
Kode Jurnal: jppertaniandd170485
