EVALUASI IN VITRO TERHADAP KEMAMPUAN ISOLAT Lactobacillus plantarum 1 DALAM MENGASIMILASI KOLESTEROL DAN MENDEKONJUGASI GARAM EMPEDU
Abstract: Several studies have
shown that lactid acid bacteria can reduce cholesterol. The aim of the study
was to evaluate the ability of Lactobacillus plantarum 1 that isolated from
spontaneous fermented soymilk in reducing cholesterol by assimilation and by
bile salt conjugation. Experimental method used to test the ability of
Lactobacillus plantarum 1 R.1.3.2, Lactobacillus plantarum 1 R.11.1.2,
Lactobacillus acidophilus FNCC 0051 and Streptococcus thermophilus FNCC 0040 to
assimilate cholesterol and to deconjugate bile salt. Cholesterol assimilation
activity was determined by measuring the difference between remaining
cholesterol in control medium and medium that inoculated by LAB. Bile salt
deconjugation ability of LAB was determined by measuring free cholic acid
released. The result showed that Lactobacillus plantarum R.11.1.2 and R.1.3.2
can assimilate cholesterol 0,56 µg/ml dan 0,48 µg/ml and can deconjugate sodium
taurocholic 0,55 µmol/ml and 0,58 µmol/ml. Lactobacillus acidophilus FNCC 0051
and Streptococcus thermophilus FNCC 0040 can assimilate cholesterol 0,38 µg/ml
dan 0,49 µg/ml and can deconjugate sodium taurocholic 0,58 µmol/ml and 0,55
µmol/ml.
Keywords: Spontaneous
fermented soymilk, lactic acid bacteria, cholesterol assimilation, Bile salt
deconjugation
Penulis: Andre Pranata,
Yusmarini, Netti Herawati
Kode Jurnal: jppertaniandd160615
