AKTIVITAS ANTIMIKROBA ISOLAT BAKTERI ASAM LAKTAT DARI KULIT ARI KACANG KEDELAI TERHADAP ESCHERICHIA COLI DAN STAPHYLOCOCCUS AUREUS
Abstract: This study aims to
determine the antimicrobial activity of lactic acid bacteria isolate from
soybean epiderm against pathogens Escherichia coli 0157 and Staphylococcus
aureus ATCC-25329. The experiment was conducted experimentally using Randomized
Complete Design (RAL) with three treatments and five replications to obtain 15
experimental units. The treatment used lactic acid bacteria isolates from
soybean epiderm namely K.12.3, K.13.1 and K.22.1 tested in petri dish that
already contained pathogenic bacteria Staphylococcus aureus ATCC-25923 and
Escherichia coli 0157. Antimicrobial activity was tested using well agar
diffusion method and the data obtained were analyzed using ANOVA and continued
with DNMRT advanced test at 5% level. The results showed that the lactic acid
bacteria of the soybean epiderm had a greater inhibition against Escherichia
coli 0157 compared with Staphylococcus aureus ATCC-25923. Isolate K.13.1 was
not significantly different in inhibiting Escherichia coli 0157 which resulted
in inhibition zone of 8.313 mm and Staphylococcus aureus ATCC-25923 with
inhibition zone of 6.960 mm. Mean while, the cell-free supernatant K.22.1
isolate significantly different in inhibiting Escherichia coli 0157 with
inhibition zone of 6.860 mm and Staphylococcus aureus ATCC-25923 resulted in
inhibition zone of 5.760 mm.
Penulis: Megawati Saputri, Evy
Rossi, Usman Pato
Kode Jurnal: jppertaniandd170127
