UJI TINGKAT KONTAMINASI EKSPLAN Centella asiatica (L.) Urban (Pegagan) DALAM KULTUR IN VITRO MELALUI PERBANDINGAN DUA METODE STERILISASI
Abstract: Research has been
conducted to determine the effect of sterilizer combination to the
contamination level of Pegagan’s (Centella asiatica L. Urban ) explant. Lamina
and petiolus were used as an explant. Sterilizer combination is divided into two groups;
Methods 1 (M1) and Methods 2 (M2). M1 consists of 0,5% Tetracycline solution,
0,3% Bayclean solution, 0,7% Dithane suspension, 0,3% Tetracycline solution,
and aquadest. Meanwhile, M2 consists of Dettol solution, alkohol 70%, 20%
Bayclean solution, 0,3% Dithane suspension, and Aquadest. The difference of
both methods were on the presences of antibiotics (Tetracycline) in M1 and desinfectans
(Bayclean) in M2. Research shows that lamina sterilized by M2 has less
contamination than M1, although the petiolus show the same level of
contamination. It is believed to be related to the structure that makes lamina
contaminated easily.
Penulis: Kartika Salam Juarna
Kode Jurnal: jpbiologidd160204
