UJI FITOKIMIA, TOKSISITAS SERTA ANTIOKSIDAN EKSTRAK PROPOLI PEMBUNGKUS MADU LEBAH Trigona Incisa DENGAN METODE 2,2-diphenyl-1-picrylhidrazyl (DPPH)
ABSTRACT: Phytochemical test,
test the mortality of larvae shrimp (Brine Shrimp Lethality Test) and test the
antioxidant activity of secondary metabolites extract of propolis Trigona
Incisa been done. Based on the test results of phytochemical screening of
secondary metabolites contained in propolis extracts showed that the crude
extract contains phenolic compounds and alkaloids. Ethanol fraction containing
alkaloids and phenolic compounds. Ethyl acetate fraction containing alkaloid
compounds. Mortality of shrimp larvae test performed to determine the toxicity
values propolis extract obtained values of 50% Lethal Concentration (LC50) at
249.6079 ppm for ethanol fraction as the most active fraction. Based on the
test of antioxidant activity with DPPH values obtained Inhibition Concentration
50% (IC50) in the crude extract of 139.47 ppm ethanol, ethanol fraction at
109.44 ppm, 91.42 ppm ethyl acetate fraction and vitamin C amounted to 59.44
ppm , It can be stated that the most active fraction is the fraction of ethyl
acetate with IC50 value of 91.42 ppm..
Penulis: Aswin Thamrin, Erwin,
Syafrizal
Kod Jurnal: jpkimiadd160209
