TEKNIK APLIKASI KONSORSIUM BAKTERI ENDOFITIK PENAMBAT N2 ASAL TUMBUHAN EKOSISTEM AIR HITAM KALIMANTAN TENGAH DALAM MENINGKATKAN PERTUMBUHAN DAN PENAMBATAN N2 TANAMAN PADI GOGO
Abstract: This study was
intended to derive an application technique of bacterial consortium obtained
from plantation black water ecosystem of Centra Kalimantan, and therefore to
increase the nitrogen fixation and growth of the upland rice. The experiments
were conducted in the Biology and Biotechnology Laboratory, Faculty of
Agriculture, Padjadjara University, Jatinangor-West Java. The preliminary
selection of inoculum source from the plantation ecosystem at black water
ecosystem of Central Kalimantan produced 12 consortia of the endophytic
bacterium that increase the growth of upland rice. Among of the 12 consortia, 2
consortia (did contribute nitrogen fixation consistently and so increase the
growth of upland rice. The two consortia were a bacterial consortium (H)
isolatedfrom the leaves of Waru plant (Dillenia pulchella) and a bacterial
consortium (I) isolatedfrom Gemor plant (Alceodaphde sp.). Threefactorial of
Complete-Random Design and triplo experiment was selected for the experimental design
of this study. First factor was consortium of endophytic nitrogen fixation
bacterial with two stages (i1 = consortium H and i2 = consortium I). The second
factor was the technique of endophytic nitrogen fixation bacteria application
with two stages (aI = rice seed soaked in the bacterial suspension for 24 hours
and a2= 1 MST old rice plant sprayed the bacterial suspension). The third
factor was turbidity (population) of endophytic nitrogen fixation bacterial
inoculum with 4 stages (Po=no inoculum control, PI=107 CFU.mtI, P2=109 CFU/mtI,
P3= 1011CFU/ml). The CFU is a Colony-Forming Unit indicating microbes in aform
of colony, initially, grew from single cell. The results of this experiment
showed that the application technique of endophytic nitrogen fixation bacteria
by soaking the upland rice seed in bacterial suspension containing 1011 CFU.mrI
for 24 hours was able to increase the growth and nitrogen fixation of the
upland rice plant. Dry weight of the plant inoculated with consortia Hand I was
respectively 0.084 and 0.097g while the nitrogenase activity of the upland rice
plant inoculated with consortia Hand I was 254.0 and 225.0 nmol g"1
DWhou,-I, respectively. With Microbact 24 E (12E/12A + 12B) and API-20 NE
methods, member of endophytic nitrogen fixation bacteriafrom consortium H was
indentified as Klebsiella pneumoniae, Pseudomonas stutzeri, Brevundimonas
vesicularis, and Burkholderia cepacia while from consortium I was Klebsiella
pneumoniae, Pseudomonas fluorescens, Burkholderia cepacia,and Acinetobacter
baumanii.
Penulis: Mieke R Setiawati,
Tualar Slmarmata, Yuyun Sumarni, Dedeh H Arief, Dwi Andreas Santosa, Hari R
Harlyadi
Kode Jurnal: jpkimiadd100240