Sintesis Alkil N-asetilglukosamina (Alkil-GlcNAc) dengan Enzim N-asetilheksosaminidase yang diisolasi dari Aspergillus sp. 501
ABSTRACT: Enzymatic Synthesis
of Alkyl N-acetylglucosamine (Alkyl-GlcNAc) by Application of Transglycosylation
Activity of β-N-Acetylhexosaminidase from Aspergillus sp. 501.
β-NAcetylhexosaminidase from Aspergillus sp. 501 has transglycosylation and
hydrolytic activity. Transglycosylation occur through transfer of
N-acetylglucosamine (GlcNAc) which washydrolyzed from
p-nitrophenyl-β-N-acetylglucosamine (pNP-GlcNAc) to various alcohols. Thetransglycosylation
product was determined by Thin Layer Chromatography and High Performance Liquid
Chromatography. Using this transglycosylation activity the novel compound of
Alkyl-GlcNAc was synthesized using N-acetylchitotriose as a donor and an ethanol
as an acceptor.
Keywords: Alkyl
N-acetylglucosamine, Transglycosylation, β-N-Acetylhexosaminidase, Aspergillus
sp. 501
Penulis: Iwan Saskiawan &
Rini Handayani
Kode Jurnal: jpbiologidd110310
