Seleksi Potensi Bakteri Probiotik Indigenus Riau sebagai Agen Biopreservatif Bahan Pangan
Abstract: The use of synthetic
preservatives is not safe for consumption and very dangerous for health.Lactic
acid bacteria (LAB) is a probiotic bacterium that can produce bacteriocins
asbiopreservatives agents. Bacteriocins have been widely applied as a food
preservative, but itscommercial availability is still low and very expensive.
This study was conducted to test the abilityof 23 isolates of local probiotic
bacteria in Riau to produce bacteriocins. LAB total population wascalculated by
Total Plate Count (TPC) method. LAB inhibition test against indicator
bacteriaStaphylococcus aureus FNCC 0047 was conducted using well diffusion
method. Bacteriocinsantimicrobial confirmatory test was performed at 100OC for
15 minutes, 30 minutes, and 60minutes. The result showed that the highest LAB
population was PSG4 (22.45 x 106 cfu/ml) andthe lowest was SLK2 (4.75 x 106
cfu/ml). The greatest inhibitory activity against indicator bacteriaS. aureus
FNCC 0047 was found in PSG2 (15.1 mm) and the smallest was in SLK2 (10.75
mm)with the activity of bacteriocins 2575 mm2/ml and 792.6 mm2/ml respectively.
The bacteriocinsconfirmatory test showed clear zone of isolates JN5, PSG2, and
SLK2 with the largest inhibitionzone was during heating for 30 minutes and 60
minutes (19.2 mm, 19.5 mm, and 16.8 mm) whilethe smallest was for 60 minutes
and 15 minutes (12.8 mm, 15.6mm, and 14.7 mm).
Penulis: NUR ASYIAH WULANDARI,
BERNADETA LENI FIBRIARTI, RODESIA MUSTIKA ROZA
Kode Jurnal: jpbiologidd160585
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