Respon Pemberian Hormon 2,4-D dan BAP terhadap Pertumbuhan Subkultur Kalus Kedelai (Glycine max (L.) Merrill) secara In Vitro
Abstrak: The purpose of this
study was to evaluate the response of administration of a combination of 2,4-D
and BAP on the growth of soybean callus subculture and determine the
combination of 2,4-D and BAP most good for the growth of soybean callus
subculture. The study design used completely randomized design (CRD) with a
pattern factorials. The factor one e.i 2,4-D consists of 4 levels, namely: 0,
5, 10, and 15 ppm. A factor of 2 e.i BAP consists of 4 levels, namely: 0, 2, 4,
dan 6 ppm. Every combination treatment repeated three times. Parameters
measured include the percentage is growing callus, type of callus, dry weight
and wet weight of soybean callus. The results showed that administration of the
hormone 2,4-D and BAP can spur the growth of soybean callus subculture, the
combination of BAP 2 ppm and 10 ppm of 2,4-D is the best combination for a
percentage of callus and growing callus types, whereas the wet weight and the
weight dried callus is not driven by a combination of the hormone 2,4-D and BAP
Penulis: Kiki Ayuningrum, Iman
Budisantoso, Kamsinah
Kode Jurnal: jpbiologidd150864
