RAPD PROFILE OF WINGED-BEAN [Psophocarpus tetragonolobus (L.) DC] MUTATED USING ETHYL-METHANE SULPHONATE
Abstract: Genetic diversity of
winged-bean [Psophocarpus tetragonolobus (L.) DC] can be produced by induced
mutation with EMS (ethyl-methane sulphonate). To identify the genetic diversity
afterEMS application on winged-bean, molecular analysis using RAPD (Randomly
AmplifiedPolymorphic DNA) technique was carried out. This study aims to
identify RAPD profile of wingedbean after EMS application. Winged-bean seeds of
both long and short pod types were soaked inwet paper towels for 6 hours
followed by soaking in EMS of 0.3% and 0.5% for 4 hours. Wingedbean seeds of
both pod types without EMS served as control. Ten of the 30 primers operon technologies
USA and 2 primers bangalore genei showed sufficiently clear bands (OPA 1, OPA
3, OPA 10, OPA 15, OPA 19, OPB 1, OPB 6, OPB 7, ES10C27, and ES10C28). All the
ten primers produced polymorphic fragments with a total number of 320 and size
ranging from 100 bp to 2,000 bp. As many as 66 loci were observed. The
polymorphism values obtained ranged from 16.67% to 46.97%. This low
polymorphism values indicate that EMS concentrations applied have not resulted in
sufficiently high genetic variation. At the same time, the genetic distance
observed were only 0.11 to 0.41.
Author: Fajri Hartanti
Journal Code: jpbiologigg160001
