PRODUKSI METABOLIT SEKUNDER STEVIOSIDA PADA KULTUR KALUS STEVIA (Stevia rebaudiana Bert. M.) DENGAN PENAMBAHAN ZPT 2,4-D DAN PEG (Polyethylene Glykol) 6000 PADA MEDIA MS (Murashige & Skoog)
Abstract: Stevia rebaudiana is
a member of the Asteraceae family, produce steviol glycosides (steviosida,
rebaudiosida A, B, C, D, E and dulcosida A) which can be used as food additives
such as food flavoring or sweetener in nutritional supplements. This study aims
to determine the concentration of 2,4-D combination and PEG 6000 were effective
in increasing the production of secondary metabolites in callus of stevia
(Stevia rebaudiana Bert. M) in vitro. PEG 6000 is a way to manipulate media in
vitro to increase secondary metabolite.
This research used Completely Randomized Design (CRD) with 12 factorial
combination with three replications. The first factor is the provision of plant
growth regulator 2,4-D (1 mg / L, 2 mg / L, and 3 mg / L) and the second factor
is the provision of PEG 6000 (0 mg / L, 5 mg / L, 15 mg / L, and 25 mg / L).
The emerging callus (day), percentage (%) of explants callus (g), weight of
callus, callus morphology (texture and color of the callus), and secondary
metabolites steviosida were measured. Data were analyzed by analysis of
variance (ANOVA) two way and to find a significant difference test Duncan's
Multiple Range Test (DMRT) at 5% significance level. Steviosida secondary
metabolites were measured using High Performance Liquid Cromatography (HPLC).
The results showed that the combination treatment of 1 mg / L 2,4-D and
25 mg / L PEG 6000 is a combination of the most efficient way to get the amount
of secondary metabolites steviosida 4.792 mg / g. Observations callus
morphology (texture and color of the callus) showed that the compact callus
textured and colored brown due to osmotic stress of PEG 6000 has a high content
of secondary metabolites steviosida
Penulis: Fittriya Nur Laila
Kode Jurnal: jpbiologidd141084
